Larvacide effectiveness of Papaya leaf extract (Carica papaya) on the mortality of larvae vector of Dengue hemorrhagic fever caused by Aedes aegypti

Abstract

Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus, which is still a public health problem in Indonesia. Chemical insecticides as larvicides used to control Aedes aegypti have created resistant populations. Thus, higher doses are needed, which, of course, have toxic effects on animals, humans, and the environment.To analyze the effectiveness of papaya leaf extract (Carica papaya) against the mortality of the third instar Aedesaegypti mosquito larvae. This study is a true experimental research design with a posttest-only control group design. In this study, the groups in this study were the larvae of the Aedes aegypti mosquito instar III/IV, papaya leaf ethanol extract, abate as a positive control group, and aquadest as a negative control.The results of the mean number of larvae deaths in each treatment group was zero (0) larvae in negative control group, positive control group (25 larvae), 5% concentration (9.5 larvae), 10% concentration (11.75 larvae), 15% concentration (12.75 larvae), a concentration of 20% (14.75 larvae), and a concentration of 25% (19.5 larvae). The results of Kruskal Wallis analysis showed that papaya leaf extract was effective as a larvicide for Aedesaegypti (p=0.001). In the probit analysis, it was found that the LC50 of the extract against Aedes aegypti was 23%, while the LC99 was 55%.Papaya leaf extract is effective as a vegetable larvicide for 3rd/IV instar Aedesaegypti larvae