Abstract
After we had applied the Semliki Forest Virus (SFV) expression system to produce large amounts of hCOX-2 in spinners, we succeeded in the first reactor runs with the SFV used and produced the serotonin receptor 5-HT3 in large quantities. We successfully scaled up the SFV technology allowing rapid gene expression at 11.5 litre scale. BHK cells were chosen as host cells for expression. They were grown in a bioreactor, equipped with a Vibromix agitation system providing for mixing and preventing cell aggregation. Medium exchange and subsequent viral infection was followed by a one day expression phase yielding 3 million of serotonin receptors per cell. After the harvest the, receptor protein could be purified in a single step at high yield (50%).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
