Abstract
It is unknown if the sex difference in fetal lung surfactant is due to synthesis or secretion. To address this we studied the lamellar body(LB) content of fetal rabbit lung at 27-30d of gestation. LBs were isolated from sex-specific lung homogenates by centrifugation on discontinuous sucrose density gradients (Longmuir et al, Arch Biochem Biophys 212:491,1981). Phosphatidylcholine(PC), saturated PC(SPC), sphingomyelin, phosphatidyl-glycerol,-inositol,-serine, and -ethanolamine were separated by one- and two-dimension thin layer chromotography and measured by phosphorus assay. Marked increases with gestation were noted, reflecting accumulation of LBs with development. At 27 and 28 days females had more total LB phospholipid/gm lung wgt and 10-25% more of each component, for example (nanomoles/gm lung wgt, mean±SE):
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