Abstract
Preparations from the flowers or herb of the white dead nettle (Lamium album L.) are recommended for the treatment of upper respiratory tract disorders or as a topical medication for mild inflammation of the throat, mouth, and skin. Taking into consideration the significance of L. album in traditional medicines across Europe, as well as the lack of studies describing the quantities of their most abundant constituents, we aimed to design a high-performance liquid chromatography coupled with diode-array detection (HPLC–DAD) method for potential standardization procedures of extracts from flowers of L. album. The HPLC–DAD method was developed and validated for quantification of iridoids (lamalbid), phenolic acids/depsides (chlorogenic acid), phenylpropanoids (verbascoside), and flavonoids (rutin; quercetin malonylhexoside; tiliroside) in aqueous and ethanolic-aqueous extracts of Lamii albi flos. The method was specific, accurate, and precise. Lamalbid was the most abundant compound both in aqueous (39.09 ± 1.02 m/g dry weight) and ethanolic-aqueous (26.66 ± 0.64 m/g dry weight) extracts. The quantities of selected compounds, except for chlorogenic acid and tiliroside, were higher in the aqueous extract than in the ethanolic-aqueous one. In conclusion, the method developed allowed for quantitation of compounds from different classes. In particular, chlorogenic acid and verbascoside have been proposed as reference compounds for routine quantitative control of Lamii albi flos.
Highlights
Progress towards ensuring and promoting human health requires guarantees in the quality of both food and medicinal plant products
The Lamiales is a wide order of plants among which well-known, or economically important
(8), which chemical are usefultools, in theparticularly quantitativechlorogenic analysis of acid verbascoside plant(3), materials (Figure (6), 1). and tiliroside (8), which are useful in the quantitative analysis of plant materials (Figure 1)
Summary
Progress towards ensuring and promoting human health requires guarantees in the quality of both food and medicinal plant products. In the latter case, quality specifications are usually provided in the international pharmacopoeias, which do not include a large number of herbal products. Quality specifications are usually provided in the international pharmacopoeias, which do not include a large number of herbal products Nowadays, this particular case is widely discussed [1,2]. The tests designed for the quality control of neglected plants are still required in the developing states of Europe [1]. There is still a need to ensure the quality of traditionally used medicinal products by using modern control techniques and applying suitable
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