Abstract
Lipase from Pseudomonas cepacia was coupled with 2,4-bis[ O-methoxypoly(ethylene glycol)]-6-chloro- s-triazine (activated PEG 2) to form PEG-modified lipase. The PEG-lipase becomes soluble and active in organic solvents. 16-Hexadecanolide was synthesized with PEG-lipase from 16-hydroxyhexadecanoic acid ethyl ester via intramolecular ester exchange reaction. The lactone was effectively obtained in high concentrations of the substrate; the yield was 92% at 1 mM, 48% at 10 mM and 11% at 100 mM substrate.
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