Abstract
1. 1. The possibility of natural hybridization between Etheostoma spectrabile and E. caeruleum at two Ohio locations was investigated using starch gel electrophoresis. Fifty-six fish, including eight which were morphologically intermediate, were analyzed at 26 presumptive genetic loci. 2. 2. Although key morphological characteristics indicated that both species occurred in both streams, electrophoretic data demonstrated the presence of only E. caeruleum in one stream. 3. 3. No biochemical evidence for hybridization between E. spectabile and E. caeruleum was found. 4. 4. Etheostoma caeruleum appears to be morphologically plastic and the key taxonomic characteristics used for its field identification may not be valid.
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