Abstract
Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS)-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems.
Highlights
Over the last decade, development of integrated and inexpensive point-of-care (POC) devices for rapid diagnostics has been on the rise owing to miniaturization and automation of analytical protocols based on microfluidics
Amongst numerous microfluidic technologies available, this study proposes the design of a centrifugal microfluidic chip, known as Lab-on-a-Disc (LOD), which is regarded as one of the most outstanding platforms in microfluidics [15,16,17,18,19]
Disc Fabrication was and Structure used as the main material in the fabrication of the disc in order to withstand the heat generated during the lysis heating process
Summary
Development of integrated and inexpensive point-of-care (POC) devices for rapid diagnostics has been on the rise owing to miniaturization and automation of analytical protocols based on microfluidics. The success of microfluidics in POC applications largely depends upon integration of the principal operation onto a monolithic device. The transition from simple independent microfluidic devices to a complete microfluidic POC system capable of performing the fundamental processes of bio-assay protocol is challenging. Specific to POC applications, improvements to present systems are required to ensure proper integration of microfluidic functions and reduction of fabrication costs; this is in addition to the necessary adaptation of bio-assays to a microfluidic format. Called cell disruption, is a process that breaks cell membranes open thereby facilitating access to intracellular substances, such as DNA, proteins, and other components for further analysis [5,6]. As the first procedure of cell pretreatment, cell lysis plays a crucial role in obtaining intracellular components, the quality of which directly influences subsequent DNA extraction and amplification [3,7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
