Label-free single cell kinetics of the invasion of spheroidal colon cancer cells through 3D Matrigel.

Abstract

This article reports label-free, real-time, and single-cell quantification of the invasion of spheroidal colon cancer cells through three-dimensional (3D) Matrigel using a resonant waveguide grating (RWG) imager. This imager employs a time-resolved swept wavelength interrogation scheme to monitor cell invasion and adhesion with a temporal resolution up to 3 s and a spatial resolution of 12 μm. As the model system, spheroids of human colorectal adenocarcinoma HT-29 cells are generated by culturing the cells in 96-well round-bottom ultralow attachment plates. 3D Matrigel is formed by its gelation in 384-well RWG biosensor microplates. The invasion and adhesion of spheroidal HT29 cells is initiated by placing individual spheroids onto the Matrigel-coated biosensors. The time series RWG images are obtained and used to extract the optical signatures arising from the adhesion after the cells are dissociated from the spheroids and invade through the 3D Matrigel. Compound profiling shows that epidermal growth factor accelerates cancer cell invasion, while vandetanib, a multitarget kinase inhibitor, dose-dependently inhibits invasion. This study demonstrates that the label-free imager can monitor in real-time the invasion of spheroidal cancer cells through 3D matrices.