Abstract
The rate of citric acid production by Aspergillus niger B-64-5 in sucrose-mineral medium was studied in shake culture using a two-stage fermentation process. The optimum sucrose concentration for product formation was 30–100 g/l. At sucrose concentrations up to 30 g/l and above 100 g/l there was respectively a limitation and an inhibition of citric acid production. The addition of citric acid at concentrations above 10 g/l had an inhibiting effect on citric acid biosynthesis. The rate of citric acid production, for low mycelial concentrations, was proportional to the biomass level. Reduction in the rate of product formation per unit of biomass for high mvcelium levels was considered to be due to oxveen limitation.
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