Abstract
Abstract In order to clarify the mechanism of the shape change of human erythrocytes induced by anionic surfactants, kinetic experiments were performed using the stopped-flow technique and the conventional spectroscopic method. In the time range from 0.1 s to a few dozen minutes, four kinetic processes were observed. In the dead time of the stopped-flow apparatus (within 10 milli seconds), a decrease in the intensity of the transmitting light was observed. Based on an analysis of the dependence of the relaxation time of each process on the surfactant concentration for various reaction schemes, we propose a most plausible reaction model that includes four kinds of binding processes of the surfactant and three intramolecular processes. In the present paper, based on this model, we discuss the detailed mechanism of the shape change of the erythrocytes.
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