Abstract
As aparamyxovirus, Newcastle disease virus (NDV) has the ability to edit its P (phosphoprotein) gene to synthesize three kinds of viral protein (P, Vand W). It is technically very difficult to differentiate P, Vand W mRNAs, and little was known about NDV regulation of RNA-editing frequency. To investigate the rules of NDV RNA editing, the ratio of the P gene-derived transcripts (P, Vand W) was determined by sequencing at different time points post-infection. The results showed unstable ratio of Vand W mRNA at different time points, and the frequency of NDV editing was significantly increased at the early period of infection (P Newcastle disease virus; phosphoprotein; RNA editing; G insertion.
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