Abstract
Desulfovibrio vulgaris (Hildenborough) cells catalyzed the oxidation of hydrogen with several quinone compounds as exogenous electron acceptors, in which hydrogenase existing in the periplasmic space of the bacterial cells functioned as the enzyme to catalyze the reaction. The rates of the hydrogen oxidation and quinone reduction were analyzed by a Michaelis−Menten type equation to yield the values of the catalytic constant of a D. vulgaris cell, kB,cat, and the bimolecular reaction rate constants for hydrogen, kB,cat/KB,H, and for quinone, kB,cat/KB,Q. They were in the ranges of kB,cat = (1.1−5.3) × 107 s-1, kB,cat/KB,H = (1.8−2.2) × 1012 M-1 s-1 and kB,cat/KB,Q = (0.97−10) × 1010 M-1 s-1 for the reactions with four kinds of quinone compounds. The mass transfer process involved in the bacterial cell-catalyzed reaction was considered by a model taking account of the substrate diffusion to and through the cross-membrane channels (composed of proteins called porins) distributed in the bacterial outer membra...
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