Abstract
BackgroundEradication rate for Helicobacter pylori (H. pylori) has decreased due to antibiotic resistance. Therefore, new strategies are needed to enhance H. pylori eradication, especially for H. pylori with high antibiotic resistance. The objective of this study was to evaluate anti-H. pylori activities of constituents from key lime (Citrus aurantifolia) and their possible inhibitory effects on urease activity of H. pylori.MethodsHelicobacter pylori strain ATCC 43526 and triple drug resistant (TDR) H. pylori strains were used in this study. Urease activities of H. pylori strains were measured by ammonia colorimetrical quantification using ELISA reader. Minimum inhibitory concentrations were determined by agar dilution method for antibiotics and by modified media dilution method for each constituent of Citrus aurantifolia (C. aurantifolia).ResultsCitrus aurantifolia extract decreased the number of colonies of H. pylori strain ATCC 43526 and TDR H. pylori stains. An increasing concentration of C. aurantifolia extract attenuated urease activities of H. pylori strain ATCC 43526 and TDR H. pylori strains. Among constituents of C. aurantifolia, citral and 4-hexen-3-one were found to be able to inhibit the growth of H. pylori strain ATCC 43526 and TDR H. pylori strains. Furthermore, citral and 4-hexen-3-one inhibited urease activities of H. pylori strain ATCC 43526 and TDR H. pylori strains in a dose-dependent manner.ConclusionCitrus aurantifolia has antimicrobial effect on TDR H. pylori strains, suggesting that C. aurantifolia might have therapeutic potential to control antibiotic-resistant H. pylori strains that cause eradication failure using other antibiotics.
Highlights
Eradication rate for Helicobacter pylori (H. pylori) has decreased due to antibiotic resistance
triple drug resistant (TDR) H. pylori strains According to minimum inhibitory concentrations (MICs) data of clarithromycin, metronidazole, and levofloxacin for H. pylori in our previous study [20], TDR H. pylori strains were all resistant to clarithromycin, metronidazole, and levofloxacin
Effect of C. aurantifolia extract on growth and urease activities of H. pylori strain ATCC 43526 and TDR H. pylori strains First, we evaluated the effect of C. aurantifolia extract on the growth of standard H. pylori strain ATCC 43526 and TDR H. pylori strains
Summary
Eradication rate for Helicobacter pylori (H. pylori) has decreased due to antibiotic resistance. New strategies are needed to enhance H. pylori eradication, especially for H. pylori with high antibiotic resistance. The objective of this study was to evaluate anti-H. pylori activities of constituents from key lime (Citrus aurantifolia) and their possible inhibitory effects on urease activity of H. pylori. Helicobacter pylori (H. pylori) is known to be a major pathogen in the development of gastritis, peptic ulcer disease, gastric adenocarcinoma, and mucosa associated lymphoid tissue (MALT) lymphoma [1, 2]. H. pylori eradication is important for the management of these diseases. Eradication rate for H. pylori has decreased due to antibiotic resistance of H. pylori. A recent meta-analysis has shown that the intake of citrus fruits can reduce the incidence of gastric cancer in the area with high prevalence of H. pylori [12].
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