Abstract
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium. JBP485 also elevated the expression level of cell surface-associated mucins (Muc1/4/16) in rabbit corneal epithelium. The Schirmer tear test results indicated that JBP485 induced tear secretion in the rabbit model. Moreover, JBP485 clinically improved corneal epithelial damage in a mouse dry eye model. Thus, our data indicate that JBP485 efficiently promoted mucin and aqueous tear secretion in rabbit ocular surface epithelium and has the potential to be used as a novel treatment for DES.
Highlights
JBP485 is a dipeptide that was first isolated from placental extract (PE) as a mitogen for a kidney cell line, and it has subsequently been synthesized by chemical means[16,17]
We examined the expression of cell surface-associated mucin-related molecules (Muc1/4/16 and Galectin-3) and found that the expression levels of conjunctival epithelial cells (CjECs) treated with JBP485 tended to be slightly
Dry eye has become one of the most common ocular diseases, and particular attention has recently been focused on the treatment of Dry eye syndrome (DES)
Summary
JBP485 (cyclo-trans-4-L-hydroxyprolyl-L-serine) is a dipeptide that was first isolated from placental extract (PE) as a mitogen for a kidney cell line, and it has subsequently been synthesized by chemical means[16,17]. The findings of the corneal wound experiments unexpectedly revealed that rabbit eyes showed an increased level of tear secretion with JBP485. This led us to the interesting hypothesis that JBP485 might promote tear and mucin secretion in ocular surface epithelium and be a potential new treatment for DES. The purpose of this present study was to investigate the effect of JBP485 on tear and mucin secretion in ocular surface epithelium. Our data provides new insights into the function of JBP485 on the homeostasis of the ocular surface
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