Jannaschia faecimaris sp. nov., isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, non-motile, rod- or coccoid-shaped bacterial strain, designated HD-22(T), belonging to the class Alphaproteobacteria, was isolated from a tidal flat sediment of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain HD-22(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2-3 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HD-22(T) fell within the clade comprising species of the genus Jannaschia, clustering with the type strains of Jannaschia helgolandensis, Jannaschia donghaensis and Jannaschia rubra, with which it exhibited highest 16S rRNA gene sequence similarity (97.6-98.2%). Levels of 16S rRNA gene sequence similarity between strain HD-22(T) and the type strains of the other species of the genus Jannaschia were in the range 94.4-97.5 %. The DNA G+C content was 64.6 mol% and mean DNA-DNA relatedness values between strain HD-22(T) and the type strains of J. helgolandensis, J. donghaensis and J. rubra were 42.1, 40.1 and 27.0 %, respectively. Strain HD-22(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain HD-22(T) is distinguishable from recognized species of the genus Jannaschia. On the basis of the data presented, strain HD-22(T) is considered to represent a novel species of the genus Jannaschia, for which the name Jannaschia faecimaris sp. nov. is proposed. The type strain is HD-22(T) ( = KCTC 32179(T) = CCUG 63415(T)).