Abstract
Journal of NeurochemistryVolume 165, Issue 5 p. 623-624 ISSUE INFORMATIONFree Access Issue Information First published: 30 May 2023 https://doi.org/10.1111/jnc.15633 Read the full article: ‘Neuron-restrictive silencer factor/repressor element 1-silencing transcription factor (NRSF/REST) controls spatial K+ buffering in primary cortical astrocytes’ by E. Centonze, A. Marte, M. Albini, A. Rocchi, F. Cesca, M. Chiacchiaretta, T. Floss, P. Baldelli, S. Ferroni, F. Benfenati and P. Valente (J. Neurochem. vol. 165 (5), pp. 701–721) on doi:10.1111/jnc.15755 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Front cover Neuron-restrictive silencer factor/repressor element 1 (RE1)-silencing transcription factor (NRSF/REST) is a transcriptional repressor of a large cluster of neural genes containing RE1 motifs in their promoter region. NRSF/REST is ubiquitously expressed in non-neuronal cells, including astrocytes, while it is downregulated during neuronal differentiation. While neuronal NRSF/REST homeostatically regulates intrinsic excitability and synaptic transmission, the role of the high NRSF/REST expression levels in the homeostatic functions of astrocytes is poorly understood. Here, we investigated the functional consequences of NRSF/REST deletion in primary cortical astrocytes derived from NRSF/REST conditional knockout mice (KO). We found that NRSF/REST KO astrocytes displaya markedly reduced activity of inward rectifying K+ channels subtype 4.1 (Kir4.1) underlying spatial K+ buffering, associated with decreased expression and activity of the glutamate transporter-1 (GLT-1) responsible for glutamate uptake. The data indicate that astrocytic NRSF/REST directly participates in neural circuit homeostasis by regulating intrinsic excitability and excitatory transmission and that its dysfunctions may contribute to the pathogenesis of neurological disorders. Image content GFAP immunostaining (red) in Rest-KO astrocytes bearing nuclear GFP (green) staining as reporter of Cre-transduction. Read the full article ‘Neuron-restrictive silencer factor/repressor element 1-silencing transcription factor (NRSF/REST) controls spatial K+ buffering in primary cortical astrocytes’ by E. Centonze, A. Marte, M. Albini, A. Rocchi, F. Cesca, M. Chiacchiaretta, T. Floss, P. Baldelli, S. Ferroni, F. Benfenati and P. Valente (J. Neurochem. vol. 165 (5), pp. 701–721) on doi:10.1111/jnc.15755 Volume165, Issue5June 2023Pages 623-624 RelatedInformation
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