Abstract
An isotachophoretic analyser with a scanning ultraviolet (UV) photometric detection system was constructed in order to study separation processes. A fused-silica capillary (I.D. 0.53 mm, O.D. 0.66 mm) was scanned over a length of 32 cm with an assembly of a UV lamp and a detector. The assembly was driven by a linear head with a stepping motor. One scanning cycle took 7.027 s and 5333 photometric signals (a UV position spectrum) were acquired. The practical resolution was 0.15 mm. The detection limit of picric acid was 20 pmol. Using the apparatus, the separation of a four-component mixture (SPADNS, monochloroacetic acid, picric acid and acetic acid) was studied using different electrolyte systems and the effect of the pH of leading electrolyte on the separation efficiency is briefly discussed.
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