Abstract
Biological activity of (E)-azastilbenes and their numerous derivatives appears in antimicrobial and anticancer effects. They also show the behavior of liquid crystals and the ability of formation of complex compounds. In this work are presented the conditions of optimum separation and determination of chosen isomers of (E)-azastilbenes by the isotachophoretic method. In the process of optimization, the length of steps of analysis, intensity (it was justified by the fact of dependence of ion mobility on electric field intensity), and pH of solutions of electrolytes and samples were changed. A new terminating electrolyte was used for the determinations. By a process of trial and error it was proven that optimum pH for analyzed isomers are 3.8. The shortest time of analysis was obtained for individual isomers. However, for mixtures of isomers the optimum time of analysis was twice as long.
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