Abstract
A modification of the centrifugation-shell vial technique was used to isolate spotted fever group (SFG) rickettsias from triturated ticks which had previously been stored at −80 °C for up to 7 months. SFG rickettsias were successfully isolated in Vero cells from all 7 haemolymph positive ticks ( Amblyomma hebraeum) used in the experiment. Attempts were also made to use these tick triturates to establish SFG rickettsial infections in tissue culture. Vero cells were used for 5 of the tick triturates and human embryonic lung cells (HEL) for the remaining 2. Infections were successfully established in 4 of the 5 Vero cell cultures, but in none of the HEL fibroblast cultures.
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