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Abstract
A method has been developed for the isolation from single tumor tissue samples of soluble nucleotides, several nucleic acid species, and protein using a detergent-phenol technique. This method yields soluble nucleotides free of macromolecules, “soluble” RNA fractions free of high molecular weight RNA and of DNA, “ribosomal” RNA fractions free of DNA and largely free of low molecular weight RNA, DNA fractions free of RNA, protein fractions free of nucleotide materials, and DNA-bound RNA as an hydrolysate. This method is suitable for the analysis of large numbers of samples, and avoids or minimizes most of the problems inherent in existing methods.
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