Abstract
Ten microsatellite loci were developed for the grassland shrub Pimelea spinescens. Locus amplification varied between the two subspecies. For P. spinescens subsp. spinescens, nine loci amplified and were polymorphic. Number of alleles per locus ranged from two to eleven (average = 5.7) and expected heterozygosity ranged from 0.103 to 0.782. For P. spinescens subsp. pubiflora, seven loci amplified, of which only three were polymorphic. For polymorphic loci, number of alleles ranged from four to seven (average = 5.3) and expected heterozygosity ranged from 0.381 to 0.783. These loci will be used to investigate population genetic structure, assisting management of this endangered species.
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