Abstract
Soluble immune complexes (IC) circulating in the blood stream can be detected by a variety of methods and these axe widely used in clinical immunology to aid the study of immunopathology of diseases, the assessment of disease progression and the patient's response to therapy (e.g., Pussell et al., 1978; Hay et al., 1979; extensively reviewed by Theofilopoulos and Dixon, 1979). Most of these methods show the presence of IC, some provide semi-quantitative estimates but they do not identify the constituent antigen and antibody in the complexes {Lambert et al., 1978). If the antigen or the specificity of the antibody could be identified some indication of the aetiology of the disease and a pathological role for the complexes might be obtained. Moreover there are instances where IC are found in sera but neither free antigen nor antibody can be detected and in these patients the isolation and identification of the IC might be of diagnostic importance (Levo et al., 1977; Kazatchkine et al., 1980; Roberts and Lewis, 1980). In this review we discuss methods for the isolation and purification of immune complexes from biological fluids in amounts sufficient to analyse.
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