Abstract
AbstractSeparating individual proteins from complex mixtures of molecules is the basis of many biochemical investigations. The method describes the separation of immunoglobulin Y (IgY) from chicken eggs using a series of physical and chemical separation techniques. The separation is rapid, and the success of each step is readily viewed on an SDS‐polyacrylamide gel. IgY identity can be confirmed on a Western blot probed with enzyme‐labeled anti‐IgY antibodies. The method is a good illustration of protein separation when there is no enzyme activity to follow.
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