Abstract
A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests.
Highlights
The purpose of this work was to develop a rapid and sensitive high performance mode were optimized with different rates of mobile phase using hexane:ethanol (chromatograms not liquid chromatography (HPLC) method for the isolation of chavibetol for further investigation as shown)
Preliminary analysis of the essential oil by 1H-NMR showed the chromatography (CCC) method for the isolation of chavibetol from crude essential oil [15], there are no papers published for obtaining of this phenylpropanoid and methyleugenol by high performance liquid chromatography (HPLC), which is still uncommon
The HPLC analysis of crude essential oil obtained from P. pseudocaryophyllus leaves in analytical mode were optimized with different rates of mobile phase using hexane:ethanol
Summary
Pharmacological and biological activities of different species in the genus have been described, including potent antibacterial action [4,5,6], antifungal [7], anticonceptive, and anti-inflammatory properties [8]. Species of Pimenta are sources of the essential oil, which is biosynthesized in the specialized cells of aromatic plants. These species contain secondary metabolites with strong biological activities [9], including insecticidal action (knockdown effect), repellency, feeding, and oviposition deterrence and development inhibition [10]. The essential oil from leaves of P. pseudocaryophylus extracted in this study contained phenylpropanoids chavibetol (5-allyl-2-methoxyphenol, Figure 1) and methyleugenol
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