Abstract
By screening conidia of Pyrenopeziza brassicae which had been irradiated with uv light, mutant strains have been isolated which grow on complete, but not on minimal, medium. A supplement which allows growth of the mutant when added to minimal medium has been identified in the case of eight of the nine initial isolates. All but one of the mutant strains have been crossed to a prototrophic strain which is resistant to the fungicide benomyl. In six of the strains, the auxotrophic phenotype has been shown to segregate in the progeny as if due to a single gene difference, and independently from the fungicide resistance marker. Two of the auxotrophic strains sporulated on host tissue in an in vitro test. Mutants of this kind will be useful in studies of heterokaryon formation, in a proposed molecular genetic analysis of pathogenicity, and as general genetic markers for this species.
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