Abstract
Important features of the isolation of anaerobic microorganisms were examined. The detection limit and frequency and time for isolating several anaerobic bacteria by direct inoculation on thioglycollate medium and by a BacT/ALERT 3D automated system were established. Isolation of individual cells of anaerobic microorganisms with different tolerances to oxygen was shown to be possible by using thioglycollate growth medium to analyze the sterility of medicinal products. The minimum number of cells in a sample could be reliably determined in less time by using a BacT/ALERT 3D automated system and special growth medium.
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