Abstract
Aim: To isolate potential agarose digesting bacteria from gut of Eisenia fetida fed with agar agar as a major feed. Methodology: E. fetida was fed on agar agar for 17 days in a comprehensive vermicompost unit, and gut-washing was collected. A specially designed dilute nutrient medium containing agarose as a solidifying agent was used to grow and isolate agarose digesting bacteria. Agarose digestion was confirmed by iodine test and novel 'micro-tip’ assay. Results: A selected CFU comprising two bacterial species showed depressions on the media, indicative of agarose digestion. Subsequently, cloning based 16S rRNA gene identification revealed the two isolates to be Brevundimonas diminuta and Achromobacter sp. with 99% similarity. These two could not be separated as pure cultures, indicating a likely associative interaction. Interpretation: The present study is the first of its sort to demonstrate a probable associative interaction between Brevundimonas diminuta and Achromobacter sp. in agarose digestion, while using "Customized Earthworm Gut Enrichment System." Key words: Agarose digesters, Earthworm, Enrichment system, Gut microflora
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
