Abstract
The Rosaceae contain several natural hosts of tobacco mosaic virus (TMV), including apple (Kirkpatrick & Lindner, 1964), pear (Gilmer et al., 1966), cherry (Cilnier, 1967), plum (Albrech‐tova et al., 1974) and strawberry (Yankulova & Schmidt, 1974). In 1974 we isolated a virus, serologicalty related to TMV from rose (Hicks. 1979): the first record, we believe, of a tobamo virus in this host.Petals (150 g) from an unidentified climbing cultivar (probably Danse de Feu) with symptoms (Fig. 1) similar to rose flower break (Farrar & Frost, 1972) were homogenized in cold 0.01 M borate buffer pH 7.8 containing 0.01 M sodium ethylene diamine tetra‐acetate and 0.01 M sodium sulphite, filtered through muslin, and given one cycle of low‐ and high‐speed centrifuga‐tion. The pellet was resuspended in 2 ml of 0.01 M borate buffer pH 7.8. centrifuged at low speed, and assayed on a range of test plants, which were grown in Levington compost (Fisons Ltd) at mean temperatures of either 22° or 26°C, given supplementary lighting in winter, shaded
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