Abstract
Bunching onion (Allium fistulosum L.) is one of the most important vegetables in Japan. To establish a genetic basis for molecular breeding of bunching onion, we isolated 1,796 simple sequence repeat (SSR) clones by large- scale sequencing of SSR-enriched genomic DNA libraries. Of these, 1,331 (74.1%) contained (GT)n repeats (n > 5), while 314 (17.5%) were (GA)n-containing clones. The average number of SSR repeats was 10.5 and 10.4 in the (GT)n- and (GA)n-containing clones, respectively. In a sample of five bunching onion inbred lines, an average of 3.2 alleles were detected in the 100 SSR loci investigated, with the polymorphic information content averaging 0.55. These re- sults indicate that bunching onion SSRs are very rich sources of highly informative genetic markers.
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