Abstract

Ethyl acetate fraction of Bergenia ciliata was subjected to various chromatographic techniques such as silica gel CC, size exclusion CC and reverse phase CC, which resulted in isolation of two new compounds from this specie. Modern spectroscopic techniques such as 1H-NMR, 13C-NMR (BB and DEPT), 2D NMR, ESI and ESI-HR-MS were utilized to elucidate the structures of isolated secondary metabolites. Both the compounds A, B showed a remarkable fungicidal activity against cladosporium cladosporioides in TLC bio-autography method. Compound B showed maximum activity (90.34±0.81%) against control drug.

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