Abstract
A method is described for isolating pure and intact nucleoli from pig and young rat brain cortex using the procedure of Muramatsu and Busch slightly modified. The isolated nucleolar preparation possesses the following properties. (1) Morphological characteristics, as revealed by phase-contrast and electron microscopy, show pure and intact nucleoli. (2) RNA/DNA ratio is about 1.2–1.3. (3) Electrophoretic patterns of nucleolar RNA show evidence of high molecular RNA (45 S, 36 S, 32 S and 28 S), unlike those of extranucleolar RNA (28 S and 18 S). (4) Polyacrylamide gel electrophoresis of 8M urea buffer soluble proteins shows evidence of specific patterns. (5) The nucleolar preparation exhibits RNA synthetic activity. (6) The nucleolar preparation has some ribonuclease activity but no glutamic dehydrogenase activity.
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