Abstract
High-speed counter-current chromatography (HSCCC) was used to separate and purify two isoflavones for the first time from Hericium erinaceum (H. erinaceum) mycelium using a two-phase solvent system composed of chloroform-dichloromethane-methanol-water (4:2:3:2, v/v/v/v). These two isoflavones were identified as genistein (4′,5,7-trihydroxyisoflavone, C15H10O5) and daidzein (4′,7-dihydroxyisoflavone, C15H10O4), using infrared spectroscopy (IR), electro-spary ionisation mass (ESI-MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR spectra. About 23 mg genistein with 95.7% purity and 18 mg daidzein with 97.3% purity were isolated from 150 mg ethanolic extract of H. erinaceum mycelium. The results demonstrated that HSCCC was a feasible method to separate and purify genistein and daidzein from H. erinaceum mycelium.
Highlights
Hericium erinaceum (H. erinaceum), commonly called monkey head mushroom or lion’s mane mushroom, is a wood-rotting fungi that belong to the Hericiaceae family [1,2]
Most separation and purification methods are based on thin-layer chromatography and other chromatographic techniques based on a solid stationary phase, such as semi-preparative and preparative HPLC [17]
Genistein andtwo daidzein were isolated from two isoflavones isolated from H. erinaceum mycelium
Summary
Hericium erinaceum (H. erinaceum), commonly called monkey head mushroom or lion’s mane mushroom, is a wood-rotting fungi that belong to the Hericiaceae family [1,2]. Most separation and purification methods are based on thin-layer chromatography and other chromatographic techniques based on a solid stationary phase, such as semi-preparative and preparative HPLC [17] These methods were generally restricted to several disadvantages, including low-yielding, time-consuming, complex processing, high-cost, poor reproducibility, and irreversible adsorption [18,19]. The support, solid support, whichcause mayadsorption cause adsorption or degradation target compounds [18,21] It has been widely used widely for separation purification flavonoids, terpenes, polyphenols, it has been used forand separation and of purification ofalkaloids, flavonoids, alkaloids, terpenes, and other natural [22,23,24,25,26].
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