Abstract
Purpose: To isolate and purify sesquiterpene lactones from Ixeris sonchifolia (Bunge) Hance by highspeed counter-current chromatography (HSCCC).Methods: I. sonchifolia was extracted with water and then loaded on a glass column (10 ~ 1500 cm containing 3000g D101 macroporous resin) where various concentrations of aqueous ethanol (0, 10, 30, 50, and 95 %) were used to elute the column successively. The 50 % ethanol fraction was purified by HSCCC using a solvent system comprised of ethyl acetate: n-butanol: methanol: water (4: 6: 1: 20, v/v), and semi-preparative high performance liquid chromatography (HPLC). The chemical structures of thecomponents obtained were further confirmed by high-resolution mass spectroscopy (MS) and nuclear magnetic resonance spectroscopy (NMR).Results: Three compounds, including ixerin Z1 (0.7 mg), ixerin Z (11.4 mg), and 11, 13α-dihydroixerin Z (8.2 mg), with purity of 96.2, 98.2, and 98.4 %, respectively, were obtained from 200 mg each of the 50 % ethanol fraction.Conclusion: HSCCC is a rapid and effective method for isolating and purifying sesquiterpene lactones from I. sonchifolia.Keywords: Sesquiterpene lactones, High-speed counter-current chromatography, Ixeris sonchifolia, Ixerin, 13α-Dihydroixerin
Highlights
Ixeris sonchifolia (Bunge) Hance, commonly called kudiezi in China, which belongs to the family Compositae, is mainly distributed in Northeastern areas of China [1]
Under the highspeed counter-current chromatography (HSCCC) conditions, fractions A and B were separated from sample in a single HSCCC step within 240 min
The purities of compound 1 in fractions A was 79.5 % based on high performance liquid chromatography (HPLC) peak area percentage
Summary
Ixeris sonchifolia (Bunge) Hance, commonly called kudiezi in China, which belongs to the family Compositae, is mainly distributed in Northeastern areas of China [1]. It is widely used as a folk medicine in China for its remarkable medical effects, such as dissipating blood stasis, improving microcirculation, and antineoplastic activity [2,3,4,5]. Some conventional techniques including silica gel column chromatography have been successfully used to separate these chemicals from this plant [10]. These classic chromatography methods are complicated, inefficient, and time-consuming. An efficient and low-cost technique to obtain these compounds is required
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