Abstract
Echinacoside and acteoside were isolated and purified from Cistanche tubulosa (Schrenk) Wight by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-glacial acetic acid-water (1:1.2:0.2:2, v/v/v/v). The experiment yielded 16.9 mg of echinacoside and 5.1 mg of acteoside from 220 mg of Cistanche tubulosa (Schrenk) Wight extract. The purity of the two compounds was 99.14% and 95.04%, respectively, as determined by HPLC. Their structures were identified by MS, UV, IR, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Cistanche tubulosa (Schrenk) Wight.
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