Abstract
There is a growing interest in the issue of inoculation of rhizobacteria into the agricultural soil because this group of bacteria can increase productivity and quality of agriculturally important crop and contributes to the stability of agroecosystems. The aim of our work was to isolate and characterize of plant growth promoting traits (production of IAA, siderophores, phosphate solubilisation, antifungal activity) of rhizobacteria belonging to a group of plant growth promoting rhizobacteria (PGPR), and isolated from rhizosphere of maize (Zea mays L.) in luvisols. Quantitative representation of rhizobacteria of maize was 7.4 . 106 CFU.g-1 dry soil. A total of eleven species of maize rhizosphere where isolated and confirmed as PGPR in vitro. The all isolates showed positive indole-3-acetic acid (IAA) production ranging between 1.39 and 15.74 µg.ml-1. Seven strains (63.6 %) has been shown with low and 1 strain with intermediate solubilisation index of phosphates and the positive production of siderophores showed 7 isolates (63, 6 %). Except for the isolate KmiJP17B089, all others inhibited the growth of Sclerotinia sclerotiorum and Rhizoctonia solani by more than 50 %. In the case of Fusarium graminearum, on the other hand, we observed a very low inhibitory activity. The most active in observed traits were three isolates identified by the 16S rRNA gene sequencing and NCBI database using the BLAST resource as Bacillus altitudinis strain KmiJP17B089, Bacillus aryabhattai strain KmiJP17B090 and Bacillus megaterium strain KmiJP17B091. These results suggest the possibility to tested in vivo these Bacillus species as potential biological fertilizer to increase maize production.
Highlights
Hiltner (1904) discovered that the rhizosphere is the layer of soil influenced by the root, and is much richer in bacteria than the surrounding bulk soil
Plant growth promoting rhizobacteria are free-living soil bacteria that colonize the rhizosphere area of root and improve the ability of nutrients and in a variety of modes of action such as the production of phytohormones, phosphate solubilization and siderophore production or supression the phytopathogenic fungi (Grobelak et al, 2015)
Bacteria were isolated from the rhizosphere of maize that grown on the altitude of 199 m above sea level in University farm in the Slovak University of Agriculture (SUA) in Kolíňany (48°21 ́33 ́ ́N, 18°11 ́28 ́ ́E)
Summary
Hiltner (1904) discovered that the rhizosphere is the layer of soil influenced by the root, and is much richer in bacteria than the surrounding bulk soil. Plant growth promoting rhizobacteria are free-living soil bacteria that colonize the rhizosphere area of root and improve the ability of nutrients and in a variety of modes of action such as the production of phytohormones, phosphate solubilization and siderophore production or supression the phytopathogenic fungi (Grobelak et al, 2015). Many bacterial genera such as Pseudomonas, Bacillus, Enterobacter, Rhizobium, Bradyrhizobium, and Xanthomonas have been reported as potential phytohormones producing rhizobacteria (Asari et al, 2016) to support plant growth. The aim of our study was isolation, characterization and identification of the most active rhizobacteria from maize and in vitro testing of their PGPR traits (phosphate solubilization, production of IAA, production of siderophores and antifungal activity) for potential in enhancing the growth of a maize crop
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of microbiology, biotechnology and food sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
