Abstract
Betasatellite DNAs associated with Old World monopartite begomoviruses encode a single gene (βC1) which mediate the vital functions required for viral pathogenicity. A region of upstream translation start site of βC1 of Cotton leaf curl Multan betasatellite (CLCuMB) associated with Tomato leaf curl New Delhi virus was tested for its promoter activity. Transcript mapping studies revealed that the transcript had a very short leader sequence of 12 nt and 3′ end was 9 nt downstream of stop codon at 186 nt coordinate position. Transient expression studies in Nicotiana benthamiana using the β-glucuronidase reporter gene driven by a βC1 promoter of CLCuMB identified a 169 nt region (between 720 and 869 nt coordinate) upstream of βC1 which is important for βC1 transcription. Histochemical GUS staining of selected solanaceous hosts indicated that βC1 promoter was active in leaves of N. benthamiana and Nicotiana tabacum and leaves and fruit of tomato. Temporal regulation studies on viral transcript using semi-quantitative PCR showed that the βC1 is a late phase gene and is expressed only 48 h post-inoculation.
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