Abstract
The production of polyhydroxyalkanoates PHAs from biopolymer degrading bacteria was examined in situ by screening isolates using Sudan B Black staining process as potential PHAs detecting, and Nile Blue staining as a proof method detection. Five bacterial strains isolated from biopolymer waste buried in a garden soil were able to produce high rate of PHA. AK1P and AK2P strains demonstrated high productivity of biopolymer by converting 5% (w/v) lactose as the only carbon source to PHA during fermentation. AY2P strain converted 5% (w/v) of glucose with less PHA accumulation. The favorite temperature for those strains to produce a high rate of PHA was at 30° C.
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