Isolation and identification of fish import consumption bacteria in a fish quarantine center, focusing on the quality control and safety of fishery products at Tanjung Priok, Jakarta

Abstract

Several bacteria were found that were not classified as DIQP I or II bacteria. Every imported fish that enters the territory of Indonesia has to go through a quarantine process first. This is because the imported fish may contain the identified bacteria. This study aims to isolate and identify bacteria in several types of imported fish and to find out whether the bacteria identified are a Disease Inducing Quarantine Pest (DIQP) I or II. The bacterial identification was conducted using biochemical and molecular biology tests, including Polymerase Chain Reaction (PCR). The fish samples analyzed were 69 tails consisting of 10 imported fish species (Mackerel, Salmon, Tuna, Swordfish, Marlin, Black Cod, Oil Fish, Yellow Tail, Pacific Saury, Flounder fish). The results of the isolation and identification of the imported fish samples through the biochemical tests identified 16 types of bacteria, dominated by Aeromonas caviae, Pseudomonas aeruginosa, Proteus morganii, Proteus vulgaris, and Vibrio fluvialis. The results of the test with the Polymerase Chain Reaction obtained all negative test samples for Aeromonas salmonicida. The bacteria found in imported fish are therefore not classified as DIQP I or DIQP II.