Abstract
Controlled postprandial glucose level is an important strategy in preventing DM type 2. Inhibitors of α-glucosidase have been postulated to be useful agents in managements of DM type 2. This research aims to isolate and identify of α-glucosidase inhibitor from Aspergillus terreus F38 by liquid fermentation. The mycelium extract of A. terreus F38showed strong activity against α-glucosidase with IC 50 value of 9.65 μg/mL. Separation and purification of mycelium ectract yielded compound I (Butyrolactone III). The structure was establish on the basis of spectral analysis, according to the data obtained by NMR and LCMS-MS experiments. Compound I showed potential activity against α-glucosidase with IC 50 value of 13.87 μg/mL. Therefore, the metabolites from A . terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents.
Highlights
The number of diabetes mellitus patient increases every year all around the world including Indonesia
The metabolites from A. terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents
Optimization of the culture medium was conducted by culturing the A. terreus F38 in three different media: Potato dextrose broth (PDB) (250 g potatoes boiled in liquid for 1h, filtered and added with water up to 1 Land 2% glucose.potato malt peptone (PMP) (PDB medium added with 1.0% malt and 0.1% peptone) and Czapek-dox (CDB) (3% sucrose, 0.2% NaNO3, 0.1% K2HPO4, 0.05 MgSO4.7H2O, 0.05% KCl, 0.001% FeSO4.7H2O) incubated for 10 days in room temperature at static condition
Summary
The number of diabetes mellitus patient increases every year all around the world including Indonesia. Cardiovascular disease contributed to 30% of the total number of deaths followed by cancers (13%), and diabetes (3%) (Soewondo et al, 2013). Postprandial hyperglycemia harbors an important role in DM type 2 progresses and specific complication such as diabetic neuropathy, retinopathy, and cardiovascular diseases (Shibano, et al, 2008).controlled postprandial glucose level is an important strategy in preventing DM type 2. Siti Munasaroh against α-glucosidase (Dewi, et al, 2018) and rubrofusarin showed potential activity inhibited of mamalian α-glucosidase, which isolated from A. aculeatus cultured in potato dextrose broth (Dewi, et al, 2016). Continuing of our efforts to isolate potential a-glucosidase inhibitors from terestrial fungi, the objective of this study was to isolated active compound from A. terreus F38 which cultured in potato dextrose broth
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