Abstract
Human GD3 synthase gene consisted of five exons and span about 135 kilobases. The 5′-flanking region lacked canonical TATA and CAAT boxes, but contained SP1 binding site(s) as in rat and mouse. The promoter activity in the 5′-flanking region (−2262∼+1) became definite when SV40 enhancer was added to the reporter plasmid. Luciferase assay with deletion mutants suggested the existence of a silencer region between −2262 and −978 nt similarly with those in mouse and rat. They also commonly contained a GT/CG repeat sequence at upstream of −1200∼−1300 nt, suggesting that they form Z-type DNA, and are involved in the gene regulation.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
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