Isolation and composition of nuclei and nucleoli.

Abstract

ABSTRACT The past decade has brought about the evolution of increasingly precise methods for isolation of nuclei and nucleoli and their components including histones, acidic proteins, high molecular weight RNA, low molecular weight RNA and ribonucleoproteins. The available methods and improving methods permit the isolation of individual unique molecular species in high states of purity. Elegant studies have been made on the amino acid sequences of some of the histones and on the nucleotide sequences of some of the species of low molecular weight RNAs uniquely found in the nucleus. In addition, nucleotide sequences are being defined for fragments of high molecular weight nuclear RNA. In studies on the effects of hormones on cells, the roles of repressor and derepressor acidic nuclear proteins are now being defined. The next decade should bring extensive new information regarding nuclear structures and functions and nucleolar components and their structures and functions.