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Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches

Publication Date Jun 1, 2022

Abstract

Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of animals used.

Concepts

Characterization Of Neural Stem Cells Numbers Of Viable Cells EdU Labeling Adult Rat Brain Neural Stem Cells Culture Of Neural Stem Cells Labor Efficiency Culture Of Neural Cells Adult Neurogenic Niches Adult Rat Spinal Cord

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