Abstract
Applying PGPB (Plant Growth-Promoting Bacteria) as bioinoculant under the excessive and continuous use of chemical fertilizer, pesticide, and herbicide need to be highlighted nowadays. One of the most beneficial PGPB is potassium-solubilizing rhizobacteria. Potassium is one of the elements that plant absorbs in a large amounts along with nitrogen. Soil in various regions of Indonesia have low potassium solubility. Therefore, finding the best novel isolates contributing to potassium solubilization need to be carried out continually. The aim of this study is to find potassium-solubilizing bacteria from two different rhizospheres (banana and chili plant) in Leuwikopo Experimental Garden and a cow manure in Cattle Pen, IPB University. To obtain the potassium-solubilizing isolates, we observed and characterized the best growing isolates and measured the solubilizing zone on Alexandrov agar medium. Three out of 10 isolates were selected and tested their pathogenicity on tobacco leave. Solubilization index of three isolates from the highest to the lowest were MPK P (3.07), MPK KT (3.03), and MPK C (2.63), respectively. Necrotic on tobacco leave after isolates treatment showed negative result which means that the three isolates were not pathogenic to plant host. The isolates are recommended to be used as bioinoculant both in greenhouse and field plants which lack of potassium availability in soil.
Highlights
Penggunaan PGPB (Plant GrowthPromoting Bacteria) atau secara khusus yang berasosiasi dengan akar tanaman juga dikenal sebagai Plant growthpromoting rhizobacteria (PGPR) (Plant Growth-Promoting Rhizobacteria) sebagai bioinokulan pemacu pertumbuhan tanaman di tengah penggunaan pupuk kimia, pestisida, dan herbisida yang terus menerus dan berlebihan saat ini sangat perlu diperhatikan
The aim of this study is to find potassium-solubilizing bacteria from two different rhizospheres (banana and chili plant) in Leuwikopo Experimental Garden and a cow manure in Cattle Pen, IPB University
Pratama, D., Anas, I., & Suwarno. (2016)
Summary
Tempat penelitian dan pengambilan sampel Penelitian ini dilakukan di Laboratorium. Bioteknologi Tanah dan Lingkungan, Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, Pascasarjana Institut Pertanian Bogor mulai September hingga Desember 2019. Sampel tanah rizosfer berasal dari tanaman cabai dan pisang yang ada di kebun percobaan Leuwikopo serta kotoran ternak sapi dari kandang peternakan kampus IPB Bogor. Sampel tanah diambil dengan menggunakan soil sampler dengan kedalaman 20 cm kemudian dimasukkan ke dalam plastik dan segera disimpan di freezer di laboratorium pada suhu 4oC
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