Abstract
Two full-length and two partial cDNAs encoding sporamin A have been isolated from sweet potato tuberous roots. Sequence comparisons show that they are very similar with 94-98% homology at nucleotide level, and 80-88% at protein level. All four cDNAs possess multiple alternate polyadenylation signals in the 3' untranslated region (3'-UTR). Genomic Southern blot analysis indicates the presence of a sporamin multigene family in sweet potato. High levels of sporamin mRNAs were detected in developing tuberous roots, but they disappeared at the sprout-germinating stage. Differential expression of these genes was obvious as their mRNAs were present specifically in developing roots, rarely in stems and not in leaves.
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