Abstract
Murine spleen cells were fractionated with the aid of bovine serum albumin gradients and by free-flow electrophoresis. These procedures were carried out in order to enrich those cells which were responsive to levamisole, a drug that has been shown to potentiate the immune response. It was found that preparative free-flow electrophoresis afforded a significantly better enrichment of these cells than that obtained by the albumin gradients. The levamisole-responsive cells were located within the high-mobility region. In addition, it was observed that the cell fractions which responded to levamisole also responded to T-cell mitogens.
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