Abstract
Thirty-six dinucleotide microsatellite markers were isolated from an enriched genomic library of southern flounder, Paralichthys lethostigma. Genotypes at all 36 microsatellites conformed to Hardy–Weinberg expectations, following Bonferroni correction for multiple tests executed simultaneously; analysis with Micro-checker indicated the possibility of null alleles at two of the microsatellites. The microsatellites characterized in this study will be useful for further evaluation of southern flounder stock structure and for assaying potential genetic impacts of stock enhancement programs.
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