Abstract
Actin was isolated from myxoamoebae (the haploid vegetativephase) of Physarum polycephalum by a simple method, employing gel filtration on Sephadex G-100. This procedure will be useful for isolating both actin and myosin from the same myosin B fraction.The purified myxoamoeba actin was homogeneous electrophoretically and contained no β-actinin-like protein. In addition, myxoamoeba actin showedproperties similar to other actins; the polymerization of G-actin with 0.1 MKC1, the reduced viscosity of F-actin, the activation of myosin ATPase by actin, and the forming of long helical filaments with 0.1 M KC1 and arro-whead-like structures with heavy meromyosin.The amino acid composition of myxoamoeba actin resembled that of theplasmodium (the diploid vegetative phase) of Physarum, except that its glycine content was higher.
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