Abstract
SummaryA virus (RFV) isolated from the urine of a human renal transplant patient and grown in human embryo kidney (HEK) cell culture had properties associated with the polyoma-SV40 subgroup of papovaviruses. Virions with papovavirus capsomeric structure, 37-43 nm in diameter, contained circular, supercoiled DNA of 2.9 × 106 daltons. Density gradient analysis revealed 2 classes of particles in infected cell cultures; full virions at 1.34 g/cm3 and empty virions at 1.29 g/cm3. Both full and empty particles agglutinated human type O erythrocytes. Infectivity and hemagglutinin were resistant to chloroform. Hemagglutination-inhibition tests and plaque neutralization tests in HEK cells indicated that RFV was related to, but distinct from SV40 virus. Antibodies against RFV were present in at least 81% of 400 human adult sera and high titers of HI antibodies against RFV were present in pooled human immune globulin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.