Abstract
A nitrile-hydrolysing bacterium, identified as Isoptericola variabilis RGT01, was isolated from industrial effluent through enrichment culture technique using acrylonitrile as the carbon source. Whole cells of this microorganism exhibited a broad range of nitrile-hydrolysing activity as they hydrolysed five aliphatic nitriles (acetonitrile, acrylonitrile, propionitrile, butyronitrile and valeronitrile), two aromatic nitriles (benzonitrile and m-Tolunitrile) and two arylacetonitriles (4-Methoxyphenyl acetonitrile and phenoxyacetonitrile). The nitrile-hydrolysing activity was inducible in nature and acetonitrile proved to be the most efficient inducer. Minimal salt medium supplemented with 50mM acetonitrile, an incubation temperature of 30°C with 2% v/v inoculum, at 200rpm and incubation of 48h were found to be the optimal conditions for maximum production (2.64±0.12 U/mg) of nitrile-hydrolysing activity. This activity was stable at 30°C as it retained around 86% activity after 4h at this temperature, but was thermolabile with a half-life of 120min and 45min at 40°C and 50°C respectively.
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