Abstract
Our study aimed to determine whether a virus from fecal samples is human rotavirus (HRV), the main pathogen that causes autumn diarrhea. Specimens were obtained from the feces from children with autumn diarrhea treated at Chuiyangliu Hospital and used to infect MA104 cells, subcultured and observed by electron microscopy. RNA was extracted, cDNA was synthesized by reverse transcription, and plaque formation and hemagglutination were assessed. The cytopathic effect (CPE) were associated with the fourth passage in subculture. CPE extracts were examined by electron microscopy, which allowed us to observe the shape of HRV particles. Amplification of the VP4 gene from HRV was used to identify the viruses as group A rotavirus. The virus causes red blood cell aggregation. The virus isolate was designated as HRV CY2017.
Highlights
Our study aimed to determine whether a virus from fecal samples is human rotavirus (HRV), the main pathogen that causes autumn diarrhea
Specimens were obtained from the feces from children with autumn diarrhea treated at Chuiyangliu Hospital and used to infect MA104 cells, subcultured and observed by electron microscopy
cytopathic effect (CPE) extracts were examined by electron microscopy, which allowed us to observe the shape of HRV particles
Summary
Our study aimed to determine whether a virus from fecal samples is human rotavirus (HRV), the main pathogen that causes autumn diarrhea. Specimens were obtained from the feces from children with autumn diarrhea treated at Chuiyangliu Hospital and used to infect MA104 cells, subcultured and observed by electron microscopy. RNA was extracted, cDNA was synthesized by reverse transcription, and plaque formation and hemagglutination were assessed. The cytopathic effect (CPE) were associated with the fourth passage in subculture. CPE extracts were examined by electron microscopy, which allowed us to observe the shape of HRV particles. Amplification of the VP4 gene from HRV was used to identify the viruses as group A rotavirus. The virus causes red blood cell aggregation. The virus isolate was designated as HRV CY2017
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
